myd88 knockout mice  (Jackson Laboratory)

Journal: Vaccines

Article Title: Differential Regulation of DC Function, Adaptive Immunity, and MyD88 Dependence by MF59 and AS03-like Adjuvants

doi: 10.3390/vaccines12050531

Figure Lengend Snippet: AddaVax increases local antigen uptake in WT and MyD88 KO mice. WT and MyD88 KO mice were intramuscularly injected with AF647−OVA alone or in the presence of AddaVax or AddaS03. Muscle was collected 15 h later, followed by single-cell preparation, immunostaining, and flow cytometry analysis. Cells were first gated based on FSC and SSC and then CD11c and MHC II expression. CD11c + MHC II + cells were gated to analyze percentage of AF647−OVA + cells. ( A ) Representative dot plots of AF647−OVA + cells in CD11c + MHC II + cells in WT ( top ) and MyD88 KO mice ( bottom ). ( B ) Comparison of percentage of AF647−OVA + cells between groups. Two−way ANOVA with Tukey’s multiple comparison test was used to compare differences between groups. n = 6. *, p < 0.05; **, p < 0.01; ***, p < 0.001.

Article Snippet: MyD88 knockout (KO) mice were purchased from Jackson Laboratory (Bar Harbor, ME, USA) and self-bred for use in this study.

Techniques: Injection, Immunostaining, Flow Cytometry, Expressing, Comparison

Journal: Vaccines

Article Title: Differential Regulation of DC Function, Adaptive Immunity, and MyD88 Dependence by MF59 and AS03-like Adjuvants

doi: 10.3390/vaccines12050531

Figure Lengend Snippet: AddaS03 increases CD80 expression on muscle DCs. Muscle DCs were analyzed for surface expression of CD80. ( A ) Representative histograms of CD80 expression on muscle DCs in WT ( top ) and MyD88 KO mice ( bottom ). Arrow: Induced peak of CD80 expression. ( B ) Comparison of MFI of CD80 on muscle DCs between groups. Two−way ANOVA with Tukey’s multiple comparison test was used to compare differences between groups. n = 6. *, p < 0.05; **, p < 0.01; ***, p < 0.001.

Article Snippet: MyD88 knockout (KO) mice were purchased from Jackson Laboratory (Bar Harbor, ME, USA) and self-bred for use in this study.

Techniques: Expressing, Comparison

Journal: Vaccines

Article Title: Differential Regulation of DC Function, Adaptive Immunity, and MyD88 Dependence by MF59 and AS03-like Adjuvants

doi: 10.3390/vaccines12050531

Figure Lengend Snippet: AddaVax depends more on MyD88 to enhance OVA-induced antibody responses. WT and MyD88 KO mice were intramuscularly immunized with 10 µg OVA alone or in the presence of AddaS03 or AddaVax adjuvant or immunized with PBS to serve as a negative control. Serum anti-OVA IgG and subtype IgG1 and IgG2c antibody titer was measured 3 weeks later and shown in ( A – C ), respectively. Two-way ANOVA with Bonferroni’s post-test was used to compare differences between groups. n = 5–7. *, p < 0.05; **, p < 0.01; ***, p < 0.001. ns: not significant.

Article Snippet: MyD88 knockout (KO) mice were purchased from Jackson Laboratory (Bar Harbor, ME, USA) and self-bred for use in this study.

Techniques: Adjuvant, Negative Control

Journal: Vaccines

Article Title: Differential Regulation of DC Function, Adaptive Immunity, and MyD88 Dependence by MF59 and AS03-like Adjuvants

doi: 10.3390/vaccines12050531

Figure Lengend Snippet: AddaVax depends more on MyD88 to enhance TIV-induced HAI titers. WT and MyD88 KO mice were subjected to IM immunization of TIV (0.3 µg HA per strain) alone or in the presence of AddaVax or AddaS03 adjuvant, or PBS. Serum HAI titer against H1N1, H3N2, type B strain was measured 3 weeks later and shown in ( A – C ), respectively. Two-way ANOVA with Fisher’s LSD test was used to compare differences between groups. n = 5–7. *, p < 0.05; **, p < 0.01; ***, p < 0.001.

Article Snippet: MyD88 knockout (KO) mice were purchased from Jackson Laboratory (Bar Harbor, ME, USA) and self-bred for use in this study.

Techniques: Adjuvant

Journal: Vaccines

Article Title: Differential Regulation of DC Function, Adaptive Immunity, and MyD88 Dependence by MF59 and AS03-like Adjuvants

doi: 10.3390/vaccines12050531

Figure Lengend Snippet: AddaVax more depends on MyD88 to enhance TIV-induced protection against pdm09 viral challenges. WT and MyD88 KO mice (the same mice in ) were challenged with a lethal dose (10× LD 50 ) of mouse-adapted pdm09 viruses. Body weight change ( top ) and survival ( bottom ) were monitored daily for 14 days in WT ( A ) and MyD88 KO mice ( B ); n = 5–7. Two-way ANOVA with Fisher’s LSD test was used to compare differences in body weight loss on individual days between groups. Log-rank test with Bonferroni’s correction was used to compare differences of survival between no adjuvant and adjuvant groups. *, p < 0.05; **, p < 0.01; ***, p < 0.001. NS: not significant.

Article Snippet: MyD88 knockout (KO) mice were purchased from Jackson Laboratory (Bar Harbor, ME, USA) and self-bred for use in this study.

Techniques: Adjuvant